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发酵乳杆菌 1

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Autologous bone marrow stem cell transplantation for the treatment of ulcerative colitis complicated

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《医学前沿（英文）》 2016年第10卷第4期页码 522-526 doi: 10.1007/s11684-016-0485-4

摘要：

Ulcerative colitis (UC) is a chronic inflammatory bowel disease with continuous or recurrent symptoms. A 42-year-old male patient with intermittent diarrhea accompanied by bloody mucopurulent stools was admitted to our hospital. The diagnosis of UC was confirmed by a combination of laboratory examination, colonoscopy, and histological assay. The patient developed herpes zoster in the hospital, which challenged traditional treatments. Therefore, we performed an autologous bone marrow cells to modulate the immune system with his permission. Autologous bone marrow mononuclear cells were collected and injected locally into the bowel mucosa, and subsequently injected systemically through a peripheral vein. After the patient underwent auto bone marrow mononuclear cells transplantations twice, the patient’s symptoms were alleviated. Furthermore, he recovered from hematochezia, and his hypersensitive C reactive protein decreased. Colonoscopy results showed reduced lesions and decreased areas with bleeding and edema in the sigmoid colon and rectum. No recurrence occurred in the subsequent two years, but long-time monitoring is still necessary for the prophylaxis of colorectal cancer.

关键词： autograft bone marrow stem cells ulcerative colitis cell therapy

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Expression of STAT6 and NF-κB p65 in the colon mucosa of patients with ulcerative colitis

Rui ZHU MD, Heng FAN MD, Lin SHEN MD, Jianguo LIU BD, Jia ZHAO MM,

《医学前沿（英文）》 2009年第3卷第4期页码 475-479 doi: 10.1007/s11684-009-0086-6

摘要： The expression of signal transducer and activator of transcription 6 (STAT6) and nuclear factor-κB (NF-κB) in the colonic mucosa of patients with ulcerative colitis (UC) was examined. Real-time polymerase chain reaction and immunohistochemistry were used to detect the expression of STAT6 and NF-κB p65 at both mRNA and protein levels in the colonic mucosa of patients with UC and healthy volunteers. The results showed that the expression levels of STAT6 and NFκB p65 in the colonic mucosa of patients with UC were significantly higher than in normal controls at both mRNA and protein levels. These data suggest that STAT6 and NFκB p65 perhaps play an important role in the pathogenesis of UC and underscore the potential value of anti-UC strategies in the clinical management of this disease.

关键词： ulcerative colitis signal transducer and activator of transcription 6 (STAT6) nuclear factor-κ B p65 (NF-κ B p65)

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Jiechangrong capsule on expression of NF-κB p65 and STAT6 in the intestinal mucosa of patients with ulcerativecolitis

Heng FAN MD, Jia ZHAO MM, Lin SHEN BM, Qing TANG MD, Zhexin SHOU MM, Li LIANG BM, Yi LIAO BM, Xiaoyan CHEN BM,

《医学前沿（英文）》 2009年第3卷第4期页码 480-484 doi: 10.1007/s11684-009-0083-9

摘要： The effects of compound Sophorae Flavescentis Jiechangrong capsule (CSFJC) on the expression of nuclear factor-κB p65 (NF-κB p65) and signal transducer and activator of transcription 6 (STAT6) in the intestinal mucosa of patients with ulcerative colitis and the possible mechanism were investigated. Eighteen patients with ulcerative colitis were randomly divided into a traditional Chinese medicine (TCM) group ( = 11) treated by CSFJC and a western medicine (WM) group ( = 7) treated by Sulfasalazine tablets. The treatment duration lasted eight weeks. Before and after the treatment, the symptoms and the physical signs were observed, and the routine stool test, the colonoscopy, and pathological examination were performed in the two groups. The expression levels of NF-κBp65 and STAT6 were detected by using immunohistochemistry. The results showed that the total effective rate of the curative effectiveness in TCM and WM groups was 100% and 71.4%, respectively, and the total effective rate of colonic mucosa lesion in TCM and WM groups was 90.9% and 71.4%, respectively, with the differences being significant (all < 0.05). The total effective rate of syndromes of damp-heat blocking according to the TCM in TCM and WM groups was 90.9% and 71.4%, respectively. After the treatment, the expression of NF-κB p65 and STAT6 in the two groups was decreased, and the decrease of NF-κB p65 and STAT6 expression in TCM group was more significant than in WM group ( < 0.05). It was concluded that CSFJC can inhibit the activation and expression of NF-κB p65 and STAT6 in the intestinal mucosa of patients with ulcerative colitis, which is a possible mechanism for CSFJC treating ulcerative colitis.

关键词： colitis ulcerative compound Sophorae Flavescentis Jiechangrong capsule nuclear factor-κ B p65 (NF-κ B p65) signal transducer and activator of transcription 6 (STAT6)

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Clinical manifestations and outcomes in severe ulcerative colitis

YANG Xuesong, YAO Wei, LIU Wenbin, LI Jun, LU Yumin

《医学前沿（英文）》 2007年第1卷第2期页码 192-195 doi: 10.1007/s11684-007-0036-0

摘要： In order to evaluate the clinical manifestations and outcomes of severe ulcerative colitis (UC), we retrospectively reviewed 41 patients with severe UC from 144 consecutively hospitalized UC cases from 1988 to 2004. Data recorded included onset, symptoms, signs, laboratory results, endoscopic, radiologic and pathologic findings, the clinical treatment process and follow-up. Of these severe cases, 92.7% (38/41) had pancolitis. Clinically, 36.9% (15/41) were categorized as first onset type, 36.9% (15/41) were chronic persistent and 26.8% (11/41) were chronic recurrent. Steroids played a main role in the remission of severe UC (61.0%). Thirty-one cases (75.6%) were relieved by drug therapy. Seven cases (17.1%) progressed to the need for operation. An early age of onset, pancolitis, low hemoglobin and serum albumin levels, and the need for intravenous steroids tended to be associated with the need for surgery. In conclusion, most of the severe UC patients respond well to drug therapy, but for individuals who are unresponsive to drug therapy, or for those depending on steroids, after a reasonable duration of treatment, the necessity for surgery should be considered.

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基于系统发育和比较基因组分析揭示与发酵乳杆菌缓解结肠炎相关的关键基因 Article

赵岩, 张程程, 于雷雷, 田丰伟, 赵建新, 张灏, 陈卫, 翟齐啸

《工程（英文）》 2022年第17卷第10期页码 170-182 doi: 10.1016/j.eng.2020.09.016

摘要：

越来越多的研究表明，发酵乳杆菌可以用于溃疡性结肠炎的预防和治疗。本研究中，我们从中国不同地区的人群粪便样本中分离出了105 株发酵乳杆菌，并对其基因组草图进行了测序。我们分析了这些菌株的泛基因组和系统发育特征，并对4 个模型菌株（发酵乳杆菌3872、CECT5716、IFO3956 和VRI003）也进行了分析。系统发育分析表明，发酵乳杆菌基因组的进化方向与宿主的地理位置、性别、族群和年龄没有明显的关系。我们挑选了3 株来自不同的系统发育支系的发酵乳杆菌（FWXBH115、FGDLZR121和FXJCJ61）和发酵乳杆菌模式菌株CECT5716，通过构建右旋糖酐硫酸钠（DSS）诱导的结肠炎小鼠模型，
探究这几株菌的抗炎和免疫调节活性。发酵乳杆菌FXJCJ61 和CECT5716 可以通过缓解所有结肠炎相关的组织学指标，保护黏膜完整性，增加肠道短链脂肪酸（SCFA），显著减轻结肠炎，而其他两株菌未能提供类似的保护作用。发酵乳杆菌FXJCJ61 和CECT5716 的抗炎机制与核转录因子kappa-B（NF-κB）信号通路激活以及促进白细胞介素10（IL-10）的产生有关。比较基因组分析结果表明，这些有益发酵乳杆菌的抗炎作用可能与一些特定基因有关。

关键词：发酵乳杆菌结肠炎抗炎系统发育分析比较基因组分析

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肠道微生态——探索中药临床疗效的新思路 Review

陆艳蒙, 谢娇娇, 彭聪高, 王保红, 王恺岑, 李兰娟

《工程（英文）》 2019年第5卷第1期页码 40-49 doi: 10.1016/j.eng.2018.11.013

摘要：

自古以来，中药在疾病预防、症状缓解和健康改善等方面发挥了重要作用。然而，复杂的成分和尚未明确的机制阻碍了中药的广泛推广和应用。越来越多的研究表明，和人体健康息息相关的肠道微生态与中药的疗效有关，因此从肠道微生态的角度去探索中药可能是打开中药奥秘的金钥匙。肠道菌群主要通过以下4个生理途径中发挥作用：参与宿主代谢、调节系统免疫、维持胃肠道稳态以及影响脑功能和宿主行为。本文回顾了中药与慢性肝病、溃疡性结肠炎、肥胖和2型糖尿病等疾病之间的联系，从肠道微生态的角度阐明其潜在的机制。未来，我们需要进一步的研究和更完善的实验设计，以揭示中药与肠道菌群之间相互作用的具体机制，并为中药的创新研究提供新的思路。

关键词：中药肠道菌群脂肪肝溃疡性结肠炎肥胖糖尿病

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Intestinal Epithelial Axin1 Deficiency Protects Against Colitis via Altered Gut Microbiota

Shari Garrett,Yongguo Zhang,Yinglin Xia,Jun Sun,

《工程（英文）》 doi: 10.1016/j.eng.2023.06.007

摘要： Intestinal homeostasis is maintained by specialized host cells and the gut microbiota. Wnt/β-catenin signaling is essential for gastrointestinal development and homeostasis, and its dysregulation has been implicated in inflammation and colorectal cancer. Axin1 negatively regulates activated Wnt/β-catenin signaling, but little is known regarding its role in regulating host–microbial interactions in health and disease. Here, we aim to demonstrate that intestinal Axin1 determines gut homeostasis and host response to inflammation. Axin1 expression was analyzed in human inflammatory bowel disease datasets. To explore the effects and mechanism of intestinal Axin1 in regulating intestinal homeostasis and colitis, we generated new mouse models with Axin1 conditional knockout in intestinal epithelial cell (IEC; Axin1^Δ^IEC) and Paneth cell (PC; Axin1^Δ^PC) to compare with control (Axin1^LoxP; LoxP: locus of X-over, P1) mice. We found increased Axin1 expression in the colonic epithelium of human inflammatory bowel disease (IBD). Axin1^Δ^IEC mice exhibited altered goblet cell spatial distribution, PC morphology, reduced lysozyme expression, and enriched Akkermansia muciniphila (A. muciniphila). The absence of intestinal epithelial and PC Axin1 decreased susceptibility to dextran sulfate sodium-induced colitis in vivo. Axin1^Δ^IEC and Axin1^Δ^PC mice became more susceptible to dextran sulfate sodium (DSS)-colitis after cohousing with control mice. Treatment with A. muciniphila reduced DSS-colitis severity. Antibiotic treatment did not change the IEC proliferation in the Axin1^Loxp mice. However, the intestinal proliferative cells in Axin1^Δ^IEC mice with antibiotic treatment were reduced compared with those in Axin1^Δ^IEC mice without treatment. These data suggest non-colitogenic effects driven by the gut microbiome. In conclusion, we found that the loss of intestinal Axin1 protects against colitis, likely driven by epithelial Axin1 and Axin1-associated A. muciniphila. Our study demonstrates a novel role of Axin1 in mediating intestinal homeostasis and the microbiota. Further mechanistic studies using specific Axin1 mutations elucidating how Axin1 modulates the microbiome and host inflammatory response will provide new therapeutic strategies for human IBD.

关键词： Axin1 Bacteria Microbiome inflammation Inflammatory bowel disease Immunity Microbiome Paneth cells Akkermansia muciniphila Wnt